2026-06-07T23:44:48Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/717282024-09-12T17:04:06Zcom_20.500.14352_14col_20.500.14352_15

Trevissón, Bibiana Becerro De Bengoa Vallejo, Ricardo Sevillano Fernández, David González, Natalia Losa Iglesias, Marta Elena López López, Daniel Alou Cervera, Luis 2023-06-22T10:49:18Z 2023-06-22T10:49:18Z 2022-03-18 2077-0383 10.3390/jcm11061683 https://hdl.handle.net/20.500.14352/71728 https://doi.org/10.3390/jcm11061683 https://www.mdpi.com/2077-0383/11/6/1683/htm Few studies have checked the impact of the hormonal/immunological dimorphism of patients on the cellular composition of platelet-rich plasma products (PRP). Whole blood (WB) from 26 volunteers was concentrated using a device previously characterized. Platelet and white blood cell (WBC) counts in WB and PRP were compared between genders, and after the population was split into pre (≤50 years)- and post (>50 years)-menopausal ages. In WB, platelet–total WBC densities were comparable in men and women. The phagocytic cell composition differed (p ≤ 0.04). After dividing by ages, phagocytic cell discrepancies were linked to women > 50 years (p ≤ 0.05), and differences emerged in lymphocyte counts (lower in >50 years groups, within and between genders, p ≤ 0.05). In PRP, densities were significantly higher, but the PRP/WB ratios varied according to blood cell (lower for phagocytic cells) and between subjects (more favorable at a lower density of a particular blood cell in WB). This “system compensatory efficiency” reduced/reverted PRP differences in the leukocyte composition between genders/age–sex groups in WB. In PRP, neutrophils were higher in younger men than older women (p < 0.05). WB lymphocyte differences between age–sex groups persisted. Age is a more determining factor than sex in the preparation of PRP. Post-menopause, sexual dimorphism strongly influences the composition of leukocytes, also conditioned by the capture efficiency of the system. eng https://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma journal article