2026-11-05T05:57:30Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/717762024-09-23T17:12:08Zcom_20.500.14352_14col_20.500.14352_15

Romero Sánchez, Iván Ramírez García, Lorena Gracia Lor, Emma Madrid Albarrán, María Yolanda 2023-06-22T10:50:55Z 2023-06-22T10:50:55Z 2022-07-11 0308-8146 10.1016/j.foodchem.2022.133611 https://hdl.handle.net/20.500.14352/71776 https://doi.org/10.1016/j.foodchem.2022.133611 Rice is frequently contaminated with aflatoxins, that are highly toxic fungal substances and strongly involved on hepatic cancer. In this work, different extraction and clean-up methods were evaluated for the simultaneous extraction and clean-up of aflatoxins B1, B2, G1 and G2 from rice. Favourable results were obtained by using methanol – water (80:20, v/v) extraction followed by immunoaffinity columns for clean-up, with recoveries of 86–92%, standard deviations between 5 and 11%, LOD ranged between 0.09 and 0.32 µg/kg, and LOQ between 0.31 and 1.06 µg/kg. Method validation and sample analysis were performed by using HPLC-MS/MS. Nine rice samples from different origin, varieties and specific characteristics, acquired in Spanish supermarkets were analysed. In two basmati samples from the same batch aflatoxin B1 was detected at (1.62 ± 0.08) µg/kg and (0.77 ± 0.03) µg/kg, both lower than the levels established by European Regulation for aflatoxin B1 in cereals. eng https://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access Atribución-NoComercial-SinDerivadas 3.0 España Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS journal article