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      <dc:title>Microleakage assessment of CAD-CAM Cobalt-Chrome and Zirconia abutments on a conical connection dental implant: A comparative in vitro study</dc:title>
      <dc:creator>Molinero Mourelle, Pedro</dc:creator>
      <dc:creator>Roccuzzo, Andrea</dc:creator>
      <dc:creator>Yilmaz, Burak</dc:creator>
      <dc:creator>Lam, Walter Yu Hang</dc:creator>
      <dc:creator>Pow, Edmond Ho Nang</dc:creator>
      <dc:creator>Gómez Polo, Miguel Ángel</dc:creator>
      <dc:creator>Río Highsmith, Jaime Del</dc:creator>
      <dc:description>Objective: To assess the marginal and bacterial microleakage in zirconia and CADCAM or cast Co-Cr implant abutments. 
Methods: Sixty-four conical connection implants with their respective abutments were divided into four groups (Co-Cr (milled, laser-sintered, and cast) and Zirconia (milled)). All specimens were subjected to a chewing simulation and thermocycling. After aging process, specimens were submerged in a 0.2% methylene blue solution with Porphyromonas gingivalis (P.g) for 48 h. The marginal microleakage was measured using a 40× optical microscopy at the internal part of the implant, and when positive microleakage was observed, a DNA isolation with a polymerase chain reaction (PCR) test was used. The microbiological assessment was based on colony forming units (CFUs). 
Results: Thirty (47%) implant-abutments presented microleakage and the PCR was performed on those specimens (1 Zirconia, 1 Co-Cr milled, 14 Co-Cr laser-sintered and 14 cast). Seven specimens (1 Co-Cr laser-sintered and six cast) presented values below the PCR detection limit t (&lt;100 CFUs). The lowest CFUs count occurred in the Co-Cr milled group (5.17E+02 CFUs/ml) followed by zirconia (7.70E+03 CFUs/ml). The Co-Cr cast (9.39E+03 CFUs/ml) and laser-sintered (2.4E+05 CFUs/ml) groups had higher bacterial count. The CFU count comparison performed between Co-Cr cast and laser-sintered resulted in a statistically significant differences in favor of Co-CrCL (p&lt;.05).
Conclusions: The abutment material and fabrication technique affected the implantabutment microleakage. Although the CAD-CAM abutments presented favorable results, all tested groups presented microleakage.</dc:description>
      <dc:date>2023-06-22T11:05:44Z</dc:date>
      <dc:date>2023-06-22T11:05:44Z</dc:date>
      <dc:date>2022-07-12</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Molinero Mourelle, P., Roccuzzo, A., Yilmaz, B. et al. «Microleakage Assessment of CAD‐CAM Cobalt‐Chrome and Zirconia Abutments on a Conical Connection Dental Implant: A Comparative in Vitro Study». Clinical Oral Implants Research, vol. 33, n.o 9, septiembre de 2022, pp. 945-52. DOI.org (Crossref), https://doi.org/10.1111/clr.13973.</dc:identifier>
      <dc:identifier>0905-7161</dc:identifier>
      <dc:identifier>10.1111/clr.13973</dc:identifier>
      <dc:identifier>https://hdl.handle.net/20.500.14352/72098</dc:identifier>
      <dc:identifier>https://doi.org/10.1111/clr.13973</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>https://creativecommons.org/licenses/by-nc-nd/3.0/es/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución-NoComercial-SinDerivadas 3.0 España</dc:rights>
      <dc:publisher>Wiley</dc:publisher>
   </ow:Publication>
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