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oai:docta.ucm.es:20.500.14352/722102024-10-02T15:50:33Zcom_20.500.14352_14col_20.500.14352_15

Selenium Agronomic Biofortification of Durum Wheat Fertilized with Organic Products: Se Content and Speciation in Grain Carucci, Federica Moreno Martín, Gustavo Madrid-Albarrán, Yolanda Gatta, Giuseppe De Vita, Pasquale Michela Giuliani, Marcella 543 sodium selenate nitrogen sulphur Triticum durum SeMetSeCys SeMet foliar fertilization ICP-MS HPLC-ICP-MS HPLC-ESI-MS/MS Química analítica (Química) 2301 Química Analítica The biofortification of cereals is a potential solution for increasing Se levels in the human diet. Here we evaluated whether applying selenate and organic N and S sources via foliar alters Se content and species in durum wheat grain. Field trials were conducted in a Mediterranean environment in 2017–2018 and 2018–2019 on old (Cappelli and old Saragolla) and modern (Marco Aurelio and Nadif) durum wheat varieties. Four organic fertilization strategies were evaluated: the control (dry blood meal at sowing), the application of foliar S (Bio-sulphur at flag leaf) and N (liquid blood meal at the beginning of heading), and their combined use in interaction with a foliar application of selenate at the booting stage. The Se content in grain was determined using ICP-MS and its speciation throughout HPLC-ICP-MS and HPLC-ESI-MS/MS. In 2018, the lower rainfall in the ten days before the Se foliar application caused a higher Se accumulation in the grain (1.63 vs. 1.44 mg kg−1 in 2017–2018 and 2018–2019, respectively). A negative effect of organic foliar S on Se content in grain was observed (−24% with respect to the control). The modern Nadif showed the highest Se content (1.87 mg kg−1), followed by the old variety Cappelli (1.57 mg kg−1). Finally, the Se speciation analysis showed the presence of SeMet and SeMetSeCys. The organic fertilization affected only SeMet, with lower values under organic S fertilization and higher values under organic N fertilization Depto. de Química Analítica Fac. de Ciencias Químicas TRUE pub 2023-06-22T11:12:38Z 2023-06-22T11:12:38Z 2022 journal article https://hdl.handle.net/20.500.14352/72210 2073-4395 10.3390/agronomy12102492 eng Atribución 3.0 España https://creativecommons.org/licenses/by/3.0/es/ open access application/pdf MDPI