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oai:docta.ucm.es:20.500.14352/723072024-09-27T17:37:41Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Aragoneses Cazorla, Guillermo author Buendia Nacarino, M.Pilar author Mena Fernández, María Luz author Luque García, José Luis 2023-06-22T11:18:49Z 2023-06-22T11:18:49Z 2022 2079-4991 10.3390/nano12101762 https://hdl.handle.net/20.500.14352/72307 https://doi.org/10.3390/nano12101762 Silver nanoparticles (AgNPs) are currently used in many different industrial, commercial and health fields, mainly due to their antibacterial properties. Due to this widespread use, humans and the environment are increasingly exposed to these types of nanoparticles, which is the reason why the evaluation of the potential toxicity associated with AgNPs is of great importance. Although some of the toxic effects induced by AgNPs have already been shown, the elucidation of more complete mechanisms is yet to be achieved. In this sense, and since the integration of metabolomics and transcriptomics approaches constitutes a very useful strategy, in the present study targeted and untargeted metabolomics and DNA microarrays assays have been combined to evaluate the molecular mechanisms involved in the toxicity induced by 10 nm AgNPs. The results have shown that AgNPs induce the synthesis of glutathione as a cellular defense mechanism to face the oxidative environment, while inducing the depletion of relevant molecules implicated in the synthesis of important antioxidants. In addition, it has been observed that AgNPs completely impair the intracellular energetic metabolism, especially affecting the production of adenosine triphosphate (ATP) and disrupting the tricarboxylic acids cycle. It has been demonstrated that AgNPs exposure also affects the glycolysis pathway. The effect on such pathway differs depending on the step of the cycle, which a significant increase in the levels of glucose as way to counterbalance the depleted levels of ATP. eng Atribución 3.0 España A Multi-Omics Approach to Evaluate the Toxicity Mechanisms Associated with Silver Nanoparticles Exposure journal article URL https://docta.ucm.es/bitstreams/8396cd49-b3e8-41c8-b10d-ae5e3967c210/download File MD5 c93ad0b11c7f797f8ab0b1fedf271468 6488688 application/pdf nanomaterials-12-01762-v3.pdf URL https://docta.ucm.es/bitstreams/cb3bb0f8-bdd4-4a36-b215-b65687917130/download File MD5 442da7f6209cc8479bb57cab95044d8a 78527 text/plain nanomaterials-12-01762-v3.pdf.txt