2026-06-28T20:22:40Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/724512024-04-16T15:29:21Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Estévez Amado, Manuel author Montalbano, Giorgia author Gallo Cordova, Álvaro author Ovejero, Jesús G. author Izquierdo Barba, Isabel author González Ortiz, Blanca author Tomasina, Clarissa author Moroni, Lorenzo author Vallet Regí, María Dulce Nombre author Vitale-Brovarone, Chiara author Fiorilli, Sonia author 2022-01 Nowadays, there is an ever-increasing interest in the development of systems able to guide and influence cell activities for bone regeneration. In this context, we have explored for the first time the combination of type-I collagen and superparamagnetic iron oxide nanoparticles (SPIONs) to design magnetic and biocompatible electrospun scaffolds. For this purpose, SPIONs with a size of 12 nm were obtained by thermal decomposition and transferred to an aqueous medium via ligand exchange with dimercaptosuccinic acid (DMSA). The SPIONs were subsequently incorporated into type-I collagen solutions to prove the processability of the resulting hybrid formulation by means of electrospinning. The optimized method led to the fabrication of nanostructured scaffolds composed of randomly oriented collagen fibers ranging between 100 and 200 nm, where SPIONs resulted distributed and embedded into the collagen fibers. The SPIONs-containing electrospun structures proved to preserve the magnetic properties of the nanoparticles alone, making these matrices excellent candidates to explore the magnetic stimuli for biomedical applications. Furthermore, the biological assessment of these collagen scaffolds confirmed high viability, adhesion, and proliferation of both preosteoblastic MC3T3-E1 cells and human bone marrow-derived mesenchymal stem cells (hBM-MSCs). 2079-4991 10.3390/nano12020181 https://hdl.handle.net/20.500.14352/72451 https://doi.org/10.3390/nano12020181 https://www.ucm.es/valletregigroup https://www.mdpi.com/2079-4991/12/2/181/html Incorporation of Superparamagnetic Iron Oxide Nanoparticles into Collagen Formulation for 3D Electrospun Scaffolds