2026-06-28T10:12:12Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/730002025-03-18T14:46:40Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Gamella Carballo, María author Laza, Anabel author Parrón Ballesteros, Jorge author Bueno Díaz, Cristina author Ruiz Valdepeñas Montiel, Víctor author Pedrero Muñoz, María author Bertolino, Franco author Pingarrón Carrazón, José Manuel author Villalba Díaz, María Teresa author Campuzano Ruiz, Susana 2023-06-22T12:39:24Z 2023-06-22T12:39:24Z 2023 Gamella, Maria, et al. «First PCR-Free Electrochemical Bioplatform for the Detection of Mustard Sin a 1 Protein as a Potential “Hidden” Food Allergen». Bioelectrochemistry, vol. 150, abril de 2023, p. 108357. DOI.org (Crossref), https://doi.org/10.1016/j.bioelechem.2022.108357. 1567-5394 10.1016/j.bioelechem.2022.108357 https://hdl.handle.net/20.500.14352/73000 https://doi.org/10.1016/j.bioelechem.2022.108357 https://www.sciencedirect.com/science/article/pii/S1567539422003085 A disposable electrochemical PCR-free biosensor for the selective detection of a fragment encoding the protein Sin a 1, a 2S albumin considered a diagnostic marker for sensitization to mustard, is reported. The methodology is based on the formation of DNA/RNA heterohybrids by sandwich hybridization of a specific fragment of the Sin a 1 allergen coding sequence with appropriately designed RNA probes. Labeling with commercial antibodies specific to the heteroduplexes and secondary antibodies conjugated with horseradish peroxidase (HRP) was carried out onto the surface of magnetic beads (MBs). Amperometric transduction was undertaken on screen-printed electrodes using H2O2 as enzyme substrate and hydroquinone (HQ) a redox mediator. The electrochemical biosensor allows the simple and fast detection (75 min) of Sin a 1 reaching a limit of detection of 3 pM. The bioplatform was successfully applied to the analysis of the targeted Sin a 1 gene specific region using just 50 ng of non-fragmented denatured genomic DNA extracted from yellow mustard seeds. eng Atribución-NoComercial-SinDerivadas 3.0 España First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen journal article URL https://docta.ucm.es/bitstreams/15c8ccd2-7841-456e-a4aa-a1f608d57d22/download File MD5 80f630e1942baaefb1fbf1c8e18a09bd 1659664 application/pdf First_PCR-free_electrochemical_bioplatform.pdf URL https://docta.ucm.es/bitstreams/27995443-023d-423e-a6a1-56bbc7f3dcaa/download File MD5 288a8f29090af11404fbcb9fbc61dcc5 61709 text/plain 1-s2.0-S1567539422003085-main.pdf.txt URL https://docta.ucm.es/bitstreams/d7720780-3857-430c-a19f-6735311cd266/download File MD5 5302653068ccf1f38f41b90dba52a332 61639 text/plain First_PCR-free_electrochemical_bioplatform.pdf.txt