2026-06-27T23:12:25Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/731692023-08-27T22:40:00Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Alonso Español, Andrea
author
Bravo Fernández, Enrique
author
Ribeiro Vidal, Honorato José
author
Virto Ruiz, Leire
author
Herrera González, David
author
Alonso Álvarez, Bettina María
author
Sanz Alonso, Mariano
2023-06-22T12:46:46Z
2023-06-22T12:46:46Z
2023-01-25
1422-0067
10.3390/ijms24032335
https://hdl.handle.net/20.500.14352/73169
https://doi.org/10.3390/ijms24032335
https://www.mdpi.com/1422-0067/24/3/2335
In search for natural products with antimicrobial properties for use in the prevention and treatment of peri-implantitis, the purpose of this investigation was to evaluate the antimicrobial activity of curcumin and xanthohumol, using an in vitro multi-species dynamic biofilm model including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of curcumin (5 mM) and xanthohumol (100 μM) extracts, and the respective controls, were evaluated with 72-h biofilms formed over dental implants by their submersion for 60 seconds. The evaluation was assessed by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). For the data analysis, comparisons were tested applying ANOVA tests with post-hoc Bonferroni corrections to evaluate the antimicrobial activity of both extracts. With qPCR, statistically significant reductions in bacterial counts were observed for curcumin and xanthohumol, when compared to the negative control. The results with CLSM and SEM were consistent with those reported with qPCR. It was concluded that both curcumin and xanthohumol have demonstrated antimicrobial activity against the six bacterial species included in the dynamic in vitro biofilm model used.
eng
Atribución 3.0 España
The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces
journal article
URL
https://docta.ucm.es/bitstreams/cb1640fa-b0b6-4132-a743-fedb601cd2c4/download
File
MD5
44399ef63665f36141f9be708ef0d66d
7515617
application/pdf
ijms-24-02335-v2.pdf
URL
https://docta.ucm.es/bitstreams/0e67e67d-764f-4ac8-a06b-286ed20d6b28/download
File
MD5
b10cac44063e806f4ed964d8822a971f
98796
text/plain
ijms-24-02335-v2.pdf.txt