2026-06-27T15:24:30Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/731992023-08-26T00:45:19Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Maloney, Jenny G. author Molokin, Aleksey author Seguí, Raimundo author Maravilla, Pablo author Martínez Hernández, Fernando author Villalobos, Guiehdani author Tsaousis, Anastasios D. author Gentekaki, Eleni author Muñoz-Antolí, Carla author Klisiowicz, Debora R. author Oishi, Camila Y. author Toledo, Rafael author Esteban, J. Guillermo author Köster, Pamela C. author de Lucio, Aida author Dashti, Alejandro author Bailo, Begoña author Calero Bernal, Rafael author González Barrio, David author Carmena, David author Santín, Mónica 2023-06-22T12:48:13Z 2023-06-22T12:48:13Z 2022-12-23 2076-2607 10.3390/microorganisms11010046 https://hdl.handle.net/20.500.14352/73199 https://doi.org/10.3390/microorganisms11010046 https://www.mdpi.com/2076-2607/11/1/46 Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity. eng Atribución 3.0 España Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38 journal article URL https://docta.ucm.es/bitstreams/2c66f478-d645-46f1-bed8-a6d39351856d/download File MD5 88b60380a13f831463cdb14b705eb5eb 1351595 application/pdf microorganisms-11-00046 (1).pdf URL https://docta.ucm.es/bitstreams/dddec6d2-f9b8-4a06-ac3d-5dd7eaf30086/download File MD5 949763fc94631693add74f8a364d5c98 51768 text/plain microorganisms-11-00046 (1).pdf.txt