2026-06-27T22:43:06Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/734182024-09-02T18:23:34Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Alcolea Alcolea, Pedro José
author
Larraga, Jaime
author
Rodríguez-Martín, Daniel
author
Alonso, Ana
author
Loayza, Francisco
author
Rojas, José M.
author
Ruiz García, Silvia
author
Louloudes Lázaro, Andrés
author
Carlón, Ana B.
author
Sánchez Cordón, Pedro José
author
Nogales Altozano, Pablo
author
Redondo, Natalia
author
Manzano García, Miguel
author
Lozano Borregón, Daniel
author
Palomero, Jesús
author
Montoya Redondo, María Luisa
author
Vallet Regí, María Dulce Nombre
author
Martín García, Verónica
author
Sevilla, Noemí
author
Larraga, Vicente
2023-06-22T13:00:57Z
2023-06-22T13:00:57Z
2022-11-09
1664-3224
10.3389/fimmu.2022.1023255
https://hdl.handle.net/20.500.14352/73418
https://www.frontiersin.org/journals/immunology
SARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic. Notwithstanding, the high mutation rate, fundamentally in the spike glycoprotein (S), is causing the emergence of new variants. Solely
utilizing this antigen is a drawback that may reduce the efficacy of these vaccines. Herein we pre ent a DNA vaccine candidate that contains the genes encoding the S and the nucleocapsid (N) proteins implemented into the nonreplicative mammalian expression plasmid vector, pPAL. This plasmid lacks antibiotic resistance genes and contains an alternative selectable marker for
production. The S gene sequence was modified to avoid furin cleavage (Sfs). Potent humoral and cellular immune responses were observed in C57BL/6J mice vaccinated with pPAL-Sfs + pPAL-N following a prime/boost regimen by the intramuscular route applying in vivo electroporation. The immunogen fully protected K18-hACE2 mice against a lethal dose (105 PFU) of SARS-CoV-2. Viral replication was completely controlled in the lungs, brain, and heart of vaccinated mice. Therefore, pPAL-Sfs + pPAL-N is a promising DNA vaccine candidate for protection from COVID-19.
eng
Atribución 3.0 España
Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2
journal article
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(831) Front. Immunol. 13, 1023255 2022.pdf
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(831) Front. Immunol. 13, 1023255 2022.pdf.txt