2026-06-27T22:25:20Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/73592023-08-26T08:04:29Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Soto, Manuel
author
Ramírez, Laura
author
Solana, José Carlos
author
Cook, Emma C. L.
author
Hernández García, Elena
author
Requena, José María
author
Iborra Martín, Salvador
2023-06-17T08:31:26Z
2023-06-17T08:31:26Z
2021
2076-2607
10.3390/microorganisms9020363
https://hdl.handle.net/20.500.14352/7359
https://doi.org/10.3390/microorganisms9020363
https://www.mdpi.com/2076-2607/9/2/363https://www.mdpi.com/journal/microorganisms
Leishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration of the LiΔHSP70-II genetically-modified attenuated L. infantum line in preventing cutaneous leishmaniasis in mice challenged with L. amazonensis virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World Leishmania species. Vaccinated mice showed a reduction in the disease evolution due to L. amazonensis challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-Leishmania IgG2a circulating antibodies accompanied to the induction of Leishmania-driven specific IFN-γ systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-ϒ production by CD4+ and CD8+ T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate LiΔHSP70-II as a candidate for the development of human vaccines.
eng
Atribución 3.0 España
Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
journal article
URL
https://docta.ucm.es/bitstreams/a5578d1a-9797-455a-8780-0bb08080e300/download
File
MD5
0c2bbb0436418e1de738ff8b755a682f
1901577
application/pdf
microorganisms-09-00363-v2.pdf