2026-06-27T15:46:49Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/74652024-10-03T13:12:03Zcom_20.500.14352_14col_20.500.14352_15

Integration of Transcriptomics and Metabolomics to Reveal the Molecular Mechanisms Underlying Rhodium Nanoparticles-Based Photodynamic Cancer Therapy Machuca Marcos, Andrés García Calvo, Estefanía Anunciação, Daniela S. Luque García, José Luis Rhodium nanoparticles have recently been described as promising photosensitizers due to their low toxicity in the absence of near-infrared irradiation, but their high cytotoxicity when irradiated. Irradiation is usually carried out with a laser source, which allows the treatment to be localized in a specific area, thus avoiding undesirable side effects on healthy tissues. In this study, a multi-omics approach based on the combination of microarray-based transcriptomics and mass spectrometry-based untargeted and targeted metabolomics has provided a global picture of the molecular mechanisms underlying the anti-tumoral effect of rhodium nanoparticle-based photodynamic therapy. The results have shown the ability of these nanoparticles to promote apoptosis by suppressing or promoting anti- and pro-apoptotic factors, respectively, and by affecting the energy machinery of tumor cells, mainly blocking the β-oxidation, which is reflected in the accumulation of free fatty acids and in the decrease in ATP, ADP and NAD+ levels. 2023-06-17T08:34:18Z 2023-06-17T08:34:18Z 2023-06-17T08:34:18Z 2021 journal article https://hdl.handle.net/20.500.14352/7465 1999-4923 10.3390/pharmaceutics13101629 eng grants PID2020-114529RB-I00 and CTQ2017-85673-R. https://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI