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oai:docta.ucm.es:20.500.14352/76112024-04-16T14:21:56Zcom_20.500.14352_14col_20.500.14352_15

Multiscale porosity in mesoporous bioglass 3D-printed scaffolds for bone regeneration. Gómez Cerezo, María Natividad Peña López, Juan Ivanovski, Saso Arcos Navarrete, Daniel Vallet Regí, María Dulce Nombre Vaquette, Cedryck Mesoporous bioactive glasses Porosity Scaffolds Bone tissue engineering Osteogenesis Materiales 3312 Tecnología de Materiales RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete) ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete) RESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí) In order to increase the bone forming ability of MBG-PCL composite scaffold, microporosity was created in the struts of 3D-printed MBG-PCL scaffolds for the manufacturing of a construct with a multiscale porosity consisting of meso- micro- and macropores. 3D-printing imparted macroporosity while the microporosity was created by porogen removal from the struts, and the MBG particles were responsible for the mesoporosity. The scaffolds were 3D-printed using a mixture of PCL, MBG and phosphate buffered saline (PBS) particles, subsequently leached out. Microporous-PCL (pPCL) as a negative control, microporous MBG-PCL (pMBG-PCL) and non- microporous-MBG-PCL (MBG-PCL) were investigated. Scanning electron microscopy, mercury intrusion poros-imetry and micro-computed tomography demonstrated that the PBS removal resulted in the formation of mi-cropores inside the struts with porosity of around 30% for both pPCL and pMBG-PCL, with both constructs displaying an overall porosity of 8090%. In contrast, the MBG-PCL group had a microporosity of 6% and an overall porosity of 70%. Early mineralisation was found in the pMBG-PCL post-leaching out and this resulted in the formation a more homogeneous calcium phosphate layer when using a biomimetic mineralisation assay. Mechanical properties ranged from 5 to 25 MPa for microporous and non-microporous specimens, hence microporosity was the determining factor affecting compressive properties. MC3T3-E1 metabolic activity was increased in the pMBG-PCL along with an increased production of RUNX2. Therefore, the microporosity within a 3D-printed bioceramic composite construct may result in additional physical and biological benefits. Unión Europea. Horizonte 2020 Ministerio de Economía y Competitividad (MINECO) Depto. de Química en Ciencias Farmacéuticas Fac. de Farmacia TRUE inpress 2023-06-17T08:56:29Z 2023-06-17T08:56:29Z 2020-11-04 journal article https://hdl.handle.net/20.500.14352/7611 0928-4931 10.1016/j.msec.2020.111706 eng VERDI (694160) MAT2016-75611-R restricted access application/pdf Elsevier