2026-08-20T06:01:47Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/77942024-07-29T13:46:12Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Díez Orejas, Rosalía María author Casarrubios Molina, Laura author Feito Castellano, María José author Rojo, J. M. author Vallet Regí, María Dulce Nombre author Arcos Navarrete, Daniel author Portolés Pérez, María Teresa author 2021-03-20 The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorporation. Macrophages play a central role in host defense and are responsible for in vivo nanoparticle trafficking. Assessment of their defense capacity against pathogenic micro-organisms after nanoparticle uptake, is necessary to prevent infections associated with nanoparticle therapies. In this study, the effects of hollow mesoporous SiO2-CaO nanospheres labeled with fluorescein isothiocyanate (FITC-NanoMBGs) on the function of peritoneal macrophages was assessed by measuring their ability to phagocytize Candida albicans expressing a red fluorescent protein. Two macrophage/fungus ratios (MOI 1 and MOI 5) were used and two experimental strategies were carried out: a) pretreatment of macrophages with FITC-NanoMBGs and subsequent fungal infection; b) competition assays after simultaneous addition of fungus and nanospheres. Macrophage pro-inflammatory phenotype markers(CD80 expression and interleukin 6 secretion) were also evaluated. Significant decreases of CD80+ macrophage percentage and interleukin 6 secretion were observed after 30 min, indicating that the simultaneous incorporation of NanoMBG and fungus favors the macrophage non-inflammatory phenotype. The present study evidences that the uptake of these nanospheres in all the studied conditions does not alter the macrophage function. Moreover, intracellular FITC-NanoMBGs induce a transitory increase of the fungal phagocytosis by macrophages at MOI 1 and after a short time of interaction. In the competition assays, as the intracellular fungus quantity increased, the intracellular FITC-NanoMBG content decreased in a MOI- and time-dependent manner. These results have confirmed that macrophages clearly distinguish between inert material and the live yeast in a dynamic intracellular incorporation. Furthermore, macrophage phagocytosis is a critical determinant to know their functional state and a valuable parameter to study the nanomaterial / macrophages / Candida albicans interface. Díez Orejas, R. M., Casarrubios Molina, L., Feito Castellano, M. J. et al. «Effects of Mesoporous SiO2-CaO Nanospheres on the Murine Peritoneal Macrophages/Candidaalbicans Interface». International Immunopharmacology, vol. 94, mayo de 2021, p. 107457. DOI.org (Crossref), https://doi.org/10.1016/j.intimp.2021.107457. 1567-5769 10.1016/j.intimp.2021.107457 https://hdl.handle.net/20.500.14352/7794 https://doi.org/10.1016/j.intimp.2021.107457 https://www.ucm.es/valletregigroup Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface