2026-06-27T11:08:56Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/79382024-10-01T13:55:16Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Velasco Bucheli, Rodrigo author Hormigo, Daniel author Fernández-Lucas, Jesús author Torres Ayuso, Pedro author Alfaro Ureña, Yohana author Saborido Modia, Ana Isolina author Serrano Aguirre, Lara author García, José Luis author Ramón, Fernando author Acebal Sarabia, Carmen author Santos de la Sen, Antonio author Arroyo Sánchez, Miguel author De La Mata Riesco, Mª Isabel 2023-06-17T09:01:06Z 2023-06-17T09:01:06Z 2020-07-01 Electronic: 2073-4344 10.3390/catal10070730 https://hdl.handle.net/20.500.14352/7938 https://doi.org/10.3390/catal10070730 https://www.mdpi.com/2073-4344/10/7/730 Many Gram-negative bacteria produce N-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In this study, kinetic parameters of several AHLs hydrolyzed by penicillin acylase from Streptomyces lavendulae (SlPA) and aculeacin A acylase from Actinoplanes utahensis (AuAAC) have been determined. Both enzymes catalyze efficiently the amide bond hydrolysis in AHLs with different acyl chain moieties (with or without 3-oxo modification) and exhibit a clear preference for AHLs with long acyl chains (C12-HSL > C14-HSL > C10-HSL > C8-HSL for SlPA, whereas C14-HSL > C12-HSL > C10-HSL > C8-HSL for AuAAC). Involvement of SlPA and AuAAC in QQ processes was demonstrated by Chromobacterium violaceum CV026-based bioassays and inhibition of biofilm formation by Pseudomonas aeruginosa, a process controlled by QS molecules, suggesting the application of these multifunctional enzymes as quorum quenching agents, this being the first time that quorum quenching activity was shown by an aculeacin A acylase. In addition, a phylogenetic study suggests that SlPA and AuAAC could be part of a new family of actinomycete acylases, with a preference for substrates with long aliphatic acyl chains, and likely involved in QQ processes. eng Atribución 3.0 España Penicillin Acylase from Streptomyces lavendulae and Aculeacin A Acylase from Actinoplanes utahensis: Two Versatile Enzymes as Useful Tools for Quorum Quenching Processes journal article URL https://docta.ucm.es/bitstreams/febcadfb-9261-4704-a23a-ad7001eb4740/download File MD5 ca1cab2256abac07816d8cd22fbba3f3 2073927 application/pdf Velasco- Buchelli, R. et al. Penicillin Acylase from Streptomyces lavendulae......pdf