2026-06-26T15:45:42Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/82052023-08-26T10:39:33Zcom_20.500.14352_14col_20.500.14352_15

Robles-Vera, Iñaki Callejo Arranz, María Ramos, Ricardo Duarte, Juan Pérez Vizcaíno, Francisco 2023-06-17T09:06:59Z 2023-06-17T09:06:59Z 2019-10-24 2072-6643 10.3390/nu11112564 https://hdl.handle.net/20.500.14352/8205 https://doi.org/10.3390/nu11112564 https://www.mdpi.com/2072-6643/11/11/2564 Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis. eng https://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Impact of Vitamin D Deficit on the Rat Gut Microbiome journal article