2026-06-27T22:44:55Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/82052023-08-26T10:39:33Zcom_20.500.14352_14col_20.500.14352_15

Impact of Vitamin D Deficit on the Rat Gut Microbiome Robles-Vera, Iñaki Callejo Arranz, María Ramos, Ricardo Duarte, Juan Pérez Vizcaíno, Francisco Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis. 2023-06-17T09:06:59Z 2023-06-17T09:06:59Z 2023-06-17T09:06:59Z 2019-10-24 journal article https://hdl.handle.net/20.500.14352/8205 2072-6643 10.3390/nu11112564 eng SAF2016-77222-R and SAF2017-8489-R https://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI