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00925njm 22002777a 4500 dc Martínez Román, Paula author López Huertas, María Rosa author Crespo Bermejo, Celia author Arca Lafuente, Sonia author Cortegano Jimeno, María Isabel author Valle Millares, Daniel author Gaspar, María Luisa author Martín Carbonero, Luz author Domínguez Domínguez, Lourdes author Ryan Múrua, Pablo author De los Santos, Ignacio author De La Fuente-Moral, Sara author Fernández Rodríguez, Amanda author Coiras, Mayte author Briz, Verónica author 2020-07-03 Coinfection with hepatitis C virus (HCV) influences HIV reservoir size. However, it is unknown whether this coinfection also induces a higher provirus transcription. Viral transcription is promoted by synergy between cellular factors such as NF-κB and the viral regulator Tat. The impact of HCV coinfection on HIV provirus transcription was analyzed in resting (r)CD4 T+ cells (CD3+CD4+CD25-CD69-HLADR-) and rCD4 T cells-depleted PBMCs (rCD4 T- PBMCs) from a multicenter cross-sectional study of 115 cART-treated HIV patients: 42 HIV+/HCV+ coinfected individuals (HIV+/HCV+), 34 HIV+ patients with HCV spontaneous clearance (HIV+/HCV−) and 39 HIV patients (HIV+). Viral transcription was assessed in total RNA through the quantification of unspliced, single spliced, and multiple spliced viral mRNAs by qPCR. Linear correlations between viral reservoir size and viral splicing were determined. A 3-fold increase of multiple spliced transcripts in rCD4 T+ cells of HIV+/HCV+ patients was found compared to HIV+ individuals (p < 0.05). As Tat is synthesized by multiple splicing, the levels of Tat were also quantified in these patients. Significant differences in single and multiple spliced transcripts were also observed in rCD4 T- PBMCs. Levels of multiple spliced mRNAs were increased in rCD4 T+ cells isolated from HIV+/HCV+ subjects, which could indicate a higher Tat activity in these cells despite their resting state. Martínez Román, P., López Huertas, M. R., Crespo Bermejo, C. et al. «Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients». Journal of Clinical Medicine, vol. 9, n.o 7, julio de 2020, p. 2091. DOI.org (Crossref), https://doi.org/10.3390/jcm9072091. 2077-0383 10.3390/jcm9072091 https://hdl.handle.net/20.500.14352/8398 https://doi.org/10.3390/jcm9072091 https://www.mdpi.com/2077-0383/9/7/2091 Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients