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oai:docta.ucm.es:20.500.14352/84142024-09-24T13:32:42Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Prieto Castañeda, Alejandro author Martínez Caballero, Siseth author Rodríguez Agarrabeitia, Antonia author García Moreno, Inmaculada author Moya Cerero, Santiago de la author Ortiz García, María Josefa author Hermoso, Juan A. 2023-06-17T09:13:05Z 2023-06-17T09:13:05Z 2021-04-29 2576-6422 10.1021/acsabm.1c00305 https://hdl.handle.net/20.500.14352/8414 https://doi.org/10.1021/acsabm.1c00305 https://pubs.acs.org/journal/aabmcb Phasing agents enabling de novo protein structure determination at ca. 1 Å, the wavelength corresponding to the maximum intensity of the synchrotron facilities applied in biomacromolecular crystallography, have been long sought-after. The first phasing agent designed for solving native protein structures at 0.97934 Å is described herein. The agent consists of a neutral ytterbium(III)-caged complex that exhibits higher anomalous signals at shorter wavelengths when compared to the best, currently applied lanthanide-based phasing agents, all of them based on gadolinium or terbium. As a proof of principle, the complex allows determining the 3D structure of a 36 kDa protein without setting the incident beam wavelength at the metal absorption edge, the strategy followed to date to gain the strongest anomalous signal even at the expense of crystallographic resolution. The agent becomes nondisruptive to the diffraction quality of the marked crystals and allows determining accurate phases, both leading to high-quality electron-density maps that enable the full tracing of the protein structure only with one agent unit bound to the protein. The high phasing power, efficient binding to the protein, low metal−macromolecule ratio, and easy handling support the developed Yb(III) complex as the best phasing agent for X-ray crystallography of a complex biomacromolecule without using modified analogues. eng Atribución 3.0 España First Lanthanide Complex for de Novo Phasing in Native Protein Crystallography at 1 Å Radiation journal article URL https://docta.ucm.es/bitstreams/d9ca9ca7-6de5-488f-b3c7-2b5ef23ab99e/download File MD5 9a910bf1a8e6749b6e5cc7f0e3546945 5261410 application/pdf acsabm.1c00305.pdf URL https://docta.ucm.es/bitstreams/9bb109b2-317d-4ede-808a-8397466402a7/download File MD5 ce370887ed180eed89a23f762fce958e 48562 text/plain acsabm.1c00305.pdf.txt