2026-06-29T01:19:23Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/886052024-09-13T15:07:58Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Yonesi, Mahdi author Ramos, Milagros author Ramírez Castillejo, Carmen author Fernández Serra, Rocío author González Nieto, Daniel author Panetsos Petrova, Fivos author Belarra, Adrián author Chevalier Del Río, Margarita author Rojo, Francisco J. author Pérez Rigueiro, José author Guinea, Gustavo V. author 2023-05-28 Central nervous system (CNS) diseases represent an extreme burden with significant social and economic costs. A common link in most brain pathologies is the appearance of inflammatory components that can jeopardize the stability of the implanted biomaterials and the effectiveness of therapies. Different silk fibroin scaffolds have been used in applications related to CNS disorders. Although some studies have analyzed the degradability of silk fibroin in non-cerebral tissues (almost exclusively upon non-inflammatory conditions), the stability of silk hydrogel scaffolds in the inflammatory nervous system has not been studied in depth. In this study, the stability of silk fibroin hydrogels exposed to different neuroinflammatory contexts has been explored using an in vitro microglial cell culture and two in vivo pathological models of cerebral stroke and Alzheimer’s disease. This biomaterial was relatively stable and did not show signs of extensive degradation across time after implantation and during two weeks of in vivo analysis. This finding contrasted with the rapid degradation observed under the same in vivo conditions for other natural materials such as collagen. Our results support the suitability of silk fibroin hydrogels for intracerebral applications and highlight the potentiality of this vehicle for the release of molecules and cells for acute and chronic treatments in cerebral pathologies. © 2023 by the authors. Yonesi, M., Ramos, M., Ramírez Castillejo, C. et al. «Resistance to Degradation of Silk Fibroin Hydrogels Exposed to Neuroinflammatory Environments». Polymers, vol. 15, n.o 11, mayo de 2023, p. 2491. DOI.org (Crossref), https://doi.org/10.3390/polym15112491. 2073-4360 10.3390/polym15112491 https://hdl.handle.net/20.500.14352/88605 https://doi.org/10.3390/polym15112491 https://www.mdpi.com/2073-4360/15/11/2491 Resistance to Degradation of Silk Fibroin Hydrogels Exposed to Neuroinflammatory Environments