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00925njm 22002777a 4500 dc Rodríguez-Alonso, Guillermo author Toledo-Marcos, Juan author Serrano-Aguirre, Lara author Rumayor, Carlos author Pasero, Beatriz author Flores Aguilar-Amat, Aída author Saborido Modia, Ana Isolina author Hoyos Vidal, María Pilar author Hernáiz Gómez-Degano, María Josefa author De La Mata Riesco, Mª Isabel author Arroyo Sánchez, Miguel author 2023 Genome mining of Streptomyces exfoliatus DSMZ 41693 has allowed us to identify four different lipase-encoding sequences, and one of them (SeLipC) has been successfully cloned and extracellularly expressed using Rhodococcus sp. T104 as a host. SeLipC was purified by one-step hydrophobic interaction chromatography. The enzyme is a monomeric protein of 27.6 kDa, which belongs to subfamily I.7 of lipolytic enzymes according to its phylogenetic analysis and biochemical characterization. The purified enzyme shows the highest activity at 60 °C and an optimum pH of 8.5, whereas thermal stability is significantly improved when protein concentration is increased, as confirmed by thermal deactivation kinetics, circular dichroism, and differential scanning calorimetry. Enzyme hydrolytic activity using p-nitrophenyl palmitate (pNPP) as substrate can be modulated by different water-miscible organic cosolvents, detergents, and metal ions. Likewise, kinetic parameters for pNPP are: KM = 49.6 µM, kcat = 57 s−1, and kcat/KM = 1.15 × 106 s−1·M−1. SeLipC is also able to hydrolyze olive oil and degrade several polyester-type polymers such as poly(butylene succinate) (PBS), poly(butylene succinate)-co-(butylene adipate) (PBSA), and poly(ε-caprolactone) (PCL). Moreover, SeLipC can catalyze the synthesis of different sugar fatty acid esters by transesterification using vinyl laurate as an acyl donor, demonstrating its interest in different biotechnological applications. Rodríguez-Alonso, G.; Toledo-Marcos, J.; Serrano-Aguirre, L.; Rumayor, C.; Pasero, B.; Flores, A.; Saborido, A.; Hoyos, P.; Hernáiz, M.J.; de la Mata, I.; et Arroyo, M (2023) "A Novel Lipase from Streptomyces exfoliatus DSMZ 41693 for Biotechnological Applications" Int. J. Mol. Sci. 24, no. 23: 17071. https://doi.org/10.3390/ijms242317071 1422-0067 10.3390/ijms242317071 https://hdl.handle.net/20.500.14352/91305 https://doi.org/10.3390/ijms242317071 https://v2.sherpa.ac.uk/id/publication/17518 A Novel Lipase from Streptomyces exfoliatus DSMZ 41693 for Biotechnological Applications