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oai:docta.ucm.es:20.500.14352/917872024-05-14T13:54:15Zcom_20.500.14352_14col_20.500.14352_15

Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages Ocaña-Fuentes, Aurelio Señorans, Francisco Javier Reglero, Guillermo Arranz Gutiérrez, Elena María Two fractions (S1 and S2) of an oregano (Origanum vulgare) extract obtained by supercritical fluid extraction have been used to test anti-inflammatory effects on activated human THP-1 cells. The main compounds present in the supercritical extract fractions of oregano were trans-sabinene hydrate, thymol and carvacrol. Fractions toxicity was assessed using the mitochondrial-respiration-dependent 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction method for several concentrations during 24 and 48 h of incubation. Concentrations higher than 30 μg/mL of both supercritical S1 and S2 oregano fractions caused a reduction in cell viability in a dose-dependent manner. Oxidized-LDLs (oxLDLs) activated THP-1 macrophages were used as cellular model of atherogenesis and the release/secretion of cytokines (TNT-α, IL-1β, IL-6 and IL-10) and their respective mRNA expressions were quantified both in presence or absence of supercritical oregano extracts. The results showed a decrease in pro-inflammatory TNF-α, IL-1β and IL-6 cytokines synthesis, as well as an increase in the production of anti-inflammatory cytokine IL-10. These results may suggest an anti-inflammatory effect of oregano extracts and their compounds in a cellular model of atherosclerosis. 2024-01-08T10:48:22Z 2024-01-08T10:48:22Z 2024-01-08T10:48:22Z 2010-03-21 journal article https://hdl.handle.net/20.500.14352/91787 0278-6915 10.1016/J.FCT.2010.03.026 eng info:eu-repo/grantAgreement/CENIT HIGEA CEN-20072003 info:eu-repo/grantAgreement/S-505/AGR-1469 info:eu-repo/grantAgreement/CSD/2007/ 063 Ministerio de Educación FPU Beca predoctoral Ocana-Fuentes, A., Arranz-Gutierrez, E., Senorans, F. J., & Reglero, G. (2010). Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages. Food and Chemical Toxicology, 48(6), 1568-1575. http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier