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Electrochemical immunosensor for rapid and sensitive determination of estradiol Ojeda Fernández, Irene Lopez-Montero, Judith Moreno Guzmán, María Janegitz, Bruno González Cortés, Araceli Yáñez-Sedeño Orive, Paloma Pingarrón Carrazón, José Manuel This work describes the preparation of an electrochemical immunosensor for estradiol based on the surface modification of a screen printed carbon electrode with grafted p-aminobenzoic acid followed by covalent binding of streptavidin (Strept) and immobilization of biotinylated anti-estradiol (antiestradiol-Biotin). The hormone determination was performed by applying a competitive immunoassay with peroxidase-labelled estradiol (HRP–estradiol) and measurement of the amperometric response at −200 mV using hydroquinone (HQ) as redox mediator. The calibration curve for estradiol exhibited a linear range between 1 and 250 pg mL−1 (r = 0.990) and a detection limit of 0.77 pg mL−1 was achieved. Cross-reactivity studies with other hormones related with estradiol at physiological concentration levels revealed the practical specificity of the developed method for estradiol. A good reproducibility, with RSD = 5.9% (n = 8) was also observed. The operating stability of a single bioelectrode modified with anti-estradiol-Biotin-Strept was nine days when it was stored at 8 ◦C under humid conditions between measurements. The developed immunosensor was applied to the analysis of certified serum and spiked urine samples with good results 2024-01-09T08:39:39Z 2024-01-09T08:39:39Z 2012 journal article Ojeda, I., et al. «Electrochemical Immunosensor for Rapid and Sensitive Determination of Estradiol». Analytica Chimica Acta, vol. 743, septiembre de 2012, pp. 117-24. https://doi.org/10.1016/j.aca.2012.07.002. 0003-2670 10.1016/j.aca.2012.07.002 https://hdl.handle.net/20.500.14352/91916 https://doi.org/10.1016/j.aca.2012.07.002 eng (Projects CTQ2009-12650) S2009/PPQ1642 restricted access Elsevier