2026-06-28T20:34:31Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/921122025-03-18T14:29:00Zcom_20.500.14352_14col_20.500.14352_15
Plasmonic nanosensors for simultaneous quantification of multiple protein–protein binding affinities
Ahijado Guzmán, Rubén
Prasad, Janak
Rosman, Christina
Henkel, Andreas
Tome, Lydia
Schneider, Dirk
Rivas, Germán
Sönnichsen, Carsten
Most of current techniques used for the quantification of protein–protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.
2024-01-09T16:47:02Z
2024-01-09T16:47:02Z
2024-01-09T16:47:02Z
2014
journal article
https://hdl.handle.net/20.500.14352/92112
1556-276X
10.1021/nl501865p
eng
Ahijado-Guzmán, Rubén, et al. «Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities». Nano Letters, vol. 14, n.o 10, octubre de 2014, pp. 5528-32. https://doi.org/10.1021/nl501865p.
http://creativecommons.org/licenses/by-nc-nd/4.0/
restricted access
Attribution-NonCommercial-NoDerivatives 4.0 International
American Chemical Society