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oai:docta.ucm.es:20.500.14352/921122025-03-18T14:29:00Zcom_20.500.14352_14col_20.500.14352_15

Plasmonic nanosensors for simultaneous quantification of multiple protein–protein binding affinities Ahijado Guzmán, Rubén Prasad, Janak Rosman, Christina Henkel, Andreas Tome, Lydia Schneider, Dirk Rivas, Germán Sönnichsen, Carsten Most of current techniques used for the quantification of protein–protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories. 2024-01-09T16:47:02Z 2024-01-09T16:47:02Z 2014 journal article Ahijado-Guzmán, Rubén, et al. «Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities». Nano Letters, vol. 14, n.o 10, octubre de 2014, pp. 5528-32. https://doi.org/10.1021/nl501865p. 1556-276X 10.1021/nl501865p https://hdl.handle.net/20.500.14352/92112 https://doi.org/10.1021/nl501865p eng http://creativecommons.org/licenses/by-nc-nd/4.0/ restricted access Attribution-NonCommercial-NoDerivatives 4.0 International American Chemical Society