2026-06-28T15:30:36Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/924282024-09-11T23:45:39Zcom_20.500.14352_14col_20.500.14352_15

NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning Muñoz Colmenero, Ana Marta Martinez, José Luis Roca, Agustín García-Vázquez, Eva The Next Generation Sequencing methodologies are considered the next step within DNA-based methods and their applicability in different fields is being evaluated. Here, we tested the usefulness of the Ion Torrent Personal Genome Machine (PGM) in food traceability analyzing candies as a model of high processed foods, and compared the results with those obtained by PCR-cloning-sequencing (PCR-CS). The majority of samples exhibited consistency between methodologies, yielding more information and species per product from the PGM platform than PCR-CS. Significantly higher AT-content in sequences of the same species was also obtained from PGM. This together with some taxonomical discrepancies between methodologies suggest that the PGM platform is still pre-mature for its use in food traceability of complex highly processed products. It could be a good option for analysis of less complex food, saving time and cost per sample. 2024-01-11T09:09:09Z 2024-01-11T09:09:09Z 2017 journal article Muñoz-Colmenero, Marta, et al. «NGS Tools for Traceability in Candies as High Processed Food Products: Ion Torrent PGM versus Conventional PCR-Cloning». Food Chemistry, vol. 214, enero de 2017, pp. 631-36. https://doi.org/10.1016/j.foodchem.2016.07.121. 0308-8146 10.1016/j.foodchem.2016.07.121 https://hdl.handle.net/20.500.14352/92428 https://doi.org/10.1016/j.foodchem.2016.07.121 eng (FPU AP-2010-5211) (GRUPIN-2014-093) (SV13-MAPFRE-1) http://creativecommons.org/licenses/by-nc-nd/4.0/ restricted access Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier