2026-06-28T15:30:33Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/926142024-05-14T15:26:34Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Martín Sabroso, Cristina
author
Fraguas Sánchez, Ana Isabel
author
Aparicio Blanco, Juan
author
Cano-Abad, M.F.
author
Torres Suárez, Ana Isabel
2024-01-11T16:46:33Z
2024-01-11T16:46:33Z
2015-01-08
Martín-Sabroso C, Fraguas-Sánchez AI, Aparicio-Blanco J, Cano-Abad MF, Torres-Suárez AI. Critical attributes of formulation and of elaboration process of PLGA-protein microparticles. Int J Pharm 2015;480(1):27-36.
0378-5173
10.1016/j.ijpharm.2015.01.008
https://hdl.handle.net/20.500.14352/92614
1873-3476
https://doi.org/10.1016/j.ijpharm.2015.01.008
Low drug loading, burst effect during release and drug inactivation account for the main drawbacks of protein microencapsulation in poly(d,l-lactic-co-glycolic) acid (PLGA) matrix by the water-in oil-in water (W/O/W) solvent evaporation method. Thus, the current study was set to invest the critical attributes of formulation and of elaboration process which determine protein loading into microparticles as well as its further release, using albumin as protein model.
NaCl concentration in the external aqueous phase, poly(vinyl alcohol) (PVA) concentration and mostly viscosity of both the internal aqueous phase and the organic phase were critical attributes for improving drug loading, with polymer molecular weight and hydrophobicity likewise directly related to albumin loading. In such a way, when using 0.5% PVA as internal aqueous phase the highest albumin loading was achieved. Optimized microparticles exhibited a sustained in vitro release of albumin over 130 days. The influence of the microencapsulation process on albumin stability and biological activity was evaluated by carrying out cell proliferation assays on PC12 cells with albumin released from microparticles. Such assay demonstrated that the microencapsulation procedure optimized in this study did not affect the biological stability of the microencapsulated protein.
eng
Critical attributes of formulation and of elaboration process of PLGA-protein microparticles
journal article
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