2026-06-08T12:56:15Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/927492025-03-18T13:38:32Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Peltomaa, Riikka Johanna
author
Barderas Manchado, Rodrigo
author
Benito Peña, María Elena
author
Moreno Bondi, María Cruz
2024-01-12T11:20:40Z
2024-01-12T11:20:40Z
2021
1618-2642
10.1007/s00216-021-03619-7
https://hdl.handle.net/20.500.14352/92749
1618-2650
https://doi.org/10.1007/s00216-021-03619-7
https://link.springer.com/article/10.1007/s00216-021-03619-7
Antibodies are widely employed as biorecognition elements for the detection of a plethora of compounds including food and environmental contaminants, biomarkers, or illicit drugs. They are also applied in therapeutics for the treatment of several disorders. Recent recommendations from the EU on animal protection and the replacement of animal-derived antibodies by non-animal-derived ones have raised a great controversy in the scientific community. The application of recombinant antibodies is expected to achieve a high growth rate in the years to come thanks to their versatility and beneficial characteristics in comparison to monoclonal and polyclonal antibodies, such as stability in harsh conditions, small size, relatively low production costs, and batch-to-batch reproducibility. This review describes the characteristics, advantages, and disadvantages of recombinant antibodies including antigen-binding fragments (Fab), single-chain fragment variable (scFv), and single-domain antibodies (VHH) and their application in food analysis with especial emphasis on the analysis of biotoxins, antibiotics, pesticides, and foodborne pathogens. Although the wide application of recombinant antibodies has been hampered by a number of challenges, this review demonstrates their potential for the sensitive, selective, and rapid detection of food contaminants.
eng
Attribution-NonCommercial-NoDerivatives 4.0 International
Recombinant antibodies and their use for food immunoanalysis
journal article
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