2026-06-29T02:38:00Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/927592025-03-18T11:55:38Zcom_20.500.14352_14col_20.500.14352_15

Click chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serum Guerrero Irigoyen, Sara Cadanno Mendía, Dona Agüí Chicharro, María Lourdes Barderas Manchado, Rodrigo Campuzano Ruiz, Susana Yáñez-Sedeño Orive, Paloma Pingarrón Carrazón, José Manuel The first electrochemical immunosensor for the determination of CXCL7 (chemokine (C-X-C motif) ligand 7) autoimmune biomarker is reported in this work. Click chemistry-assisted antibodies immobilization was per formed by reaction of azide functionalized-multi-walled carbon nanotubes (MWCNTs) and ethynyl-IgG onto screen-printed carbon electrodes. The capture antibodies were further immobilized onto IgG-MWCNTs con jugates. After a blocking step with casein, a sandwich immunoassay was implemented involving biotinylated detector antibodies and alkaline phosphatase (AP)-streptavidin conjugate. Differential pulse voltammetry upon addition of 1-naphthylphosphate was used as the analytical readout. A linear calibration plot between 0.5 and 600 pg mL−1 CXCL7 and a LOD value of 0.1 pg mL−1 were obtained. The usefulness of the immunosensor was demonstrated by the successful analysis of serum samples from patients with rheumatoid arthritis. 2024-01-12T11:36:10Z 2024-01-12T11:36:10Z 2019 journal article Guerrero S, Cadano D, Agüí L, Barderas R, Campuzano S, Yáñez-Sedeño P, et al. Click chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serum. J Electroanal Chem. 2019 Feb 21; 837:246–53. 1572-6657 10.1016/j.jelechem.2019.02.043 https://hdl.handle.net/20.500.14352/92759 https://doi.org/10.1016/j.jelechem.2019.02.043 eng CTQ2015-70023-R CTQ2015-64402-C2-1- R TRANSNANOAVANSENS P2018/NMT-4349 http://creativecommons.org/licenses/by/4.0/ restricted access Attribution 4.0 International Elsevier