2026-06-27T22:42:10Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/930662025-03-18T13:04:53Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Wilhelm, Stefan author Del Barrio Redondo, Melisa author Heiland, Josef author Himmelstoß, Sandy author Galbán, Javier author Wolfbeis, Otto author Hirsch, Thomas 2024-01-15T12:01:31Z 2024-01-15T12:01:31Z 2014 Stefan Wilhelm, Melisa del Barrio, Josef Heiland, Sandy F. Himmelstoß, Javier Galbán, Otto S. Wolfbeis, and Thomas Hirsch ACS Applied Materials & Interfaces 2014 6 (17), 15427-15433 DOI: 10.1021/am5038643 1944-8244 10.1021/am5038643 https://hdl.handle.net/20.500.14352/93066 1944-8252 https://doi.org/10.1021/am5038643 We report on upconverting luminescent nanoparticles (UCLNPs) that are spectrally tuned such that their emission matches the absorption bands of the two most important species associated with enzymatic redox reactions. The core-shell UCLNPs consist of a β-NaYF4core doped with Yb3+/Tm3+ions and a shell of pure β-NaYF4. Upon 980 nm excitation, they display emission bands peaking at 360 and 475 nm, which is a perfect match to the absorption bands of the enzyme cosubstrate NADH and the coenzyme FAD, respectively. By exploiting these spectral overlaps, we have designed fluorescent detection schemes for NADH and FAD that are based on the modulation of the emission intensities of UCLNPs by FAD and NADH via an inner filter effect. eng Spectrally matched upconverting luminescent nanoparticles for monitoring enzymatic reactions journal article 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