2026-06-27T15:23:03Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/930672024-06-24T23:58:00Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author García-Carmona, Laura author Moreno Guzmán, María author González, María Cristina author Escarpa, Alberto 2024-01-15T12:02:00Z 2024-01-15T12:02:00Z 2017 García-Carmona L, Moreno-Guzmán M, González MC, Escarpa A. Class enzyme-based motors for “on the fly” enantiomer analysis of amino acids. Biosensors and Bioelectronics 2017;96:275–80. https://doi.org/10.1016/j.bios.2017.04.051. 0956-5663 10.1016/j.bios.2017.04.051 https://hdl.handle.net/20.500.14352/93067 1873-4235 https://doi.org/10.1016/j.bios.2017.04.051 Here, two class-enzyme motors are properly designed allowing the rapid dispersion of the class-enzyme D-amino acid oxidase (DAO) and L-amino acid oxidase (LAO) for selective “on the fly” biodetection of D and L-amino acids (AAs), respectively. The efficient movement together with the continuous release of fresh class-enzyme leads to a greatly accelerated enzymatic reaction processes without the need of external stirring or chemical and physical attachment of the enzyme. Ultra-fast detection (<2 min) and accurate quantifications of L-phenylalanine (L-Phe) in plasma and whole-blood newborns samples diagnosed with Phenylketonuria and total D-AAs in Vibrio cholera cultures are pioneer illustrated as relevant examples of each enantiomer determination. These results opens clearly novel avenues in biosensing for fast screening diagnostics, decentralized monitoring and design of future points of care. eng Class enzyme-based motors for “on the fly” enantiomer analysis of amino acids journal article 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URL https://docta.ucm.es/bitstreams/f463effb-0477-4761-b98e-16fcadde56d1/download File MD5 8736f693f816897e26e9850870250290 532026 application/pdf Analysis_of_amino_acids.pdf