2026-06-27T23:19:12Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/932332025-02-20T01:12:26Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Ortega‐García, María Victoria
author
Salguero, Francisco
author
Rodríguez Bertos, Antonio Manuel
author
Moreno, Inmaculada
author
García Benzaquén, Nerea
author
García-Seco Romero, María Teresa
author
Luz Torre, Gabriela
author
Domínguez Rodríguez, Lucas José
author
Domínguez, Mercedes
2024-01-16T07:40:40Z
2024-01-16T07:40:40Z
2019
Ortega‐García MV, Salguero FJ, Rodríguez‐Bertos A, et al. A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis). Transbound Emerg Dis. 2019;66:2474–2481. https://doi.org/10.1111/ tbed.13305
1865-1674
10.1111/tbed.13305
https://hdl.handle.net/20.500.14352/93233
1865-1682
https://doi.org/10.1111/ tbed.13305
https://pubmed.ncbi.nlm.nih.gov/31339665/
In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT) and quantitative real‐time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares. However, L. infantum DNA could not be detected by qPCR on paraffin‐embedded tissue obtained by laser capture microdissection. Using the DFA assay to diagnose L. infantum, infection may provide further insights into this disease in wild animals and may allow the precise tissue localization of L. infantum, thereby guiding follow‐up tests with more accurate qPCR.
eng
Attribution 4.0 International
A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)
journal article
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