2026-06-27T23:23:08Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/933022025-03-18T12:26:29Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Mayol Hornero, Beatriz author Díez-Sánchez, Paula author Sánchez Sánchez, Alfredo author Torre, Cristina de la author Villalonga, Anabel author Lucena-Sánchez, Elena author Sancenón, Félix author Martínez Ruiz, María Paloma author Vilela García, Diana author Martínez-Máñez, Ramón author Villalonga Santana, Reynaldo author 2021 This work describes the assembly of a novel enzyme-controlled nanomachine operated through an AND Boolean logic gate for on-command delivery. The nanodevice was constructed on Au-mesoporous silica Janus nanoparticles capped with a thiol-sensitive gate-like molecular ensemble on the mesoporous face and functionalized with glutathione reductase on the gold face. This autonomous nanomachine employed NADPH and glutathione disulfide as input chemical signals, leading to the enzymatic production of reduced glutathione that causes the disruption of the gating mechanism on the mesoporous face and the consequent payload release as an output signal. The nanodevice was successfully used for the autonomous release of doxorubicin in HeLa cancer cells and RAW 264.7 macrophage cells. Mayol, B., Diez, P., Sánchez, A., Paredes, C. D. L. T., Villalonga, A., Lucena-Sánchez, E., Sancenón, F., Martínez-Ruiz, P., Vilela, D., Martinez-Manez, R., Villalonga, R.* (2021). Glutathione disulfide-sensitive Janus nanomachine controlled by an enzymatic AND logic gate for smart delivery. Nanoscale 13: 18616-18625. 2040-3364 10.1039/d0nr08282a https://hdl.handle.net/20.500.14352/93302 2040-3372 https://doi.org/10.1039/d0nr08282a A glutathione disulfide-sensitive Janus nanomachine controlled by an enzymatic and logic gate for smart delivery