2026-06-26T20:12:27Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/93429.22025-03-18T15:37:46Zcom_20.500.14352_14col_20.500.14352_15

Maestro García-Donas, María Beatriz Velasco, Isabel Castillejo, Isabel Arevalo-Rodriguez, Miguel Cebolla, Ángel Sanz, Jesús 2024-01-23T15:29:13Z 2024-01-16T15:58:30Z 2008 Maestro, Beatriz, et al. «Affinity Partitioning of Proteins Tagged with Choline-Binding Modules in Aqueous Two-Phase Systems». Journal of Chromatography A, vol. 1208, n.o 1-2, octubre de 2008, pp. 189-96. https://doi.org/10.1016/j.chroma.2008.08.106. 0021-9673 10.1016/j.chroma.2008.08.106 https://hdl.handle.net/20.500.14352/93429.2 1873-3778 https://doi.org/10.1016/j.chroma.2008.08.106 We present a novel procedure for affinity partitioning of recombinant proteins fused to the choline-binding module C-LytA in aqueous two-phase systems containing poly(ethylene glycol) (PEG). Proteins tagged with the C-LytA module and exposed to the two-phase systems are quantitatively localized in the PEG-rich phase, whereas subsequent addition of the natural ligand choline specifically shifts their localization to the PEG-poor phase by displacement of the polymer from the binding sites. The described procedure is simple, scalable and reproducible, and has been successfully applied to the purification of four diverse proteins, resulting in high yields and purity. eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems journal article