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oai:docta.ucm.es:20.500.14352/935022025-04-03T00:12:05Zcom_20.500.14352_14col_20.500.14352_15

Aranaz Martín, Alicia Romero Martínez, Beatriz Montero Serra, Natalia Álvarez Sánchez, Julio Bezos Garrido, Javier Juan Ferré, Lucía De Mateos García, Ana Isabel Domínguez Rodríguez, Lucas José 2024-01-17T08:06:08Z 2024-01-17T08:06:08Z 2004 Aranaz, A., Romero, B., Montero, N., Alvarez, J., Bezos, J., de Juan, L., Mateos, A., & Domínguez, L. (2004). Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain. Journal of clinical microbiology, 42(11), 5388–5391. https://doi.org/10.1128/JCM.42.11.5388-5391.2004 0095-1137 10.1128/JCM.42.11.5388-5391.2004 https://hdl.handle.net/20.500.14352/93502 1098-660X https://doi.org/10.1128/JCM.42.11.5388-5391.2004 Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Spoligotyping Profile Change Caused by Deletion of a Direct Variable Repeat in a Mycobacterium tuberculosis Isogenic Laboratory Strain journal article