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oai:docta.ucm.es:20.500.14352/935902025-03-18T15:46:26Zcom_20.500.14352_14col_20.500.14352_15

A combined approach based on matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles and liquid chromatography to determine polyphenols from grape residues Gómez Mejía, Esther Hartwig Mikkelsen, Line Rosales Conrado, Noelia León González, María Eugenia de Madrid Albarrán, María Yolanda Elsevier A simple and efficient low-cost matrix solid phase dispersion (MSPD) extraction assisted by TiO2 nanopar- ticles and diatomaceous earth has been developed for the extraction of phenolic compounds from grape and grape pomace wastes. Experimental conditions for MSPD extraction were optimized by a facto- rial design and a surface response methodology. The simultaneous identification and quantification of eight main natural polyphenols (caffeic, p-coumaric, dihydroxybenzoic and gallic acid, rutin, resveratrol, quercetin and catechin) was possible by combining MSPD and capillary liquid chromatography coupled to a diode array detection and a mass simple quadrupole analyzer (cLC-DAD-MS). Good linearity and acceptable LOD (0.05–62 μg· g −1 ) and LOQ (0.2–207 μg· g −1 ) were obtained. The quantities of extracted polyphenols were within 2.4 and 333 μg· g −1, with catechin and rutin the most abundant compounds in rape pomace and grape wastes, respectively. Furthermore, considering the prospective uses of the win- ery bioresidues, the extracts have been characterised in terms of bioactive properties (several antioxidant activities and bacterial inhibition against Staphylococcus aureus, Escherichia coli and Pseudomona aerugi- nosa) and parameters such as total polyphenol and total flavonoid content. The high antioxidant activity (IC 50 5.0 ± 0.4 μg ·g −1 against DPPH radical) and antibacterial activity (2.2 ± 0.3 mg· mL −1 ) suggests that the methodology developed is efficient, rapid and promising for the extraction of phenolic compounds with potential application as bioactive ingredients in food and cosmetic industries. 2024-01-17T12:04:25Z 2024-01-17T12:04:25Z 2024-01-17T12:04:25Z 2021 journal article https://hdl.handle.net/20.500.14352/93590 0021-9673 10.1016/j.chroma.2021.462128 eng S2018/BAA-4393, AVANSECAL II-CM] CTQ 2017-83569-C2-1-R CT17/ 17- CT18/ 17 Gómez-Mejía, E; Mikkelsen, LH; Rosales-Conrado, N; León-González, ME; Madrid, Y, A combined approach based on matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles and liquid chromatography to determine polyphenols from grape residues, 2021 May 10; 1644; 462128 restricted access Elsevier