2026-06-28T04:56:35Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/936872025-03-18T11:46:54Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Pérez García, Selene author Carrión Caballo, Mar author Jimeno, Rebeca author Ortiz, Ana author González-Álvaro, Isidoro author Fernández, Julián author Pérez Gomáriz, Rosa María author Juarranz Moratilla, Yasmina 2024-01-17T18:07:18Z 2024-01-17T18:07:18Z 2013 Pérez-García, S., Carrión, M., Jimeno, R. et al. Urokinase Plasminogen Activator System in Synovial Fibroblasts from Osteoarthritis Patients: Modulation by Inflammatory Mediators and Neuropeptides. J Mol Neurosci 52, 18–27 (2014). https://doi.org/10.1007/s12031-013-0189-z 0895-8696 10.1007/s12031-013-0189-z https://hdl.handle.net/20.500.14352/93687 1559-1166 https://doi.org/10.1007/s12031-013-0189-z Plasminogen activators are specific proteolytic enzymes implicated in a variety of basic biological processes. The expression of the urokinase plasminogen activator system components is increased in some human diseases, including osteoarthritis. We sought to study the effect of two components of the inflamed synovial microenvironment on this system, IL-1β and fibronectin fragments, elucidating whether corticotropin-releasing factor (CRF) and vasoactive intestinal peptide (VIP) neuropeptides modulate it, and analyzing the physiological consequences in joint destruction by measuring matrix metalloproteinases-9 and metalloproteinases-13 levels in osteoarthritis fibroblast-like synoviocytes. We showed that IL-1β and fibronectin fragments stimulated urokinase system contributing to the perpetuation of the destructive cascade in joint. VIP modulated, even at constitutive level, this system, also counteracting the effect of both inflammatory stimuli. However, CRF seemed to be ineffective in controlling the production of these proteinases. Moreover, VIP was able to reduce the constitutive expression of matrix metalloproteinase-13 and the levels of both matrix metalloproteinases after stimulation with the pro-inflammatory stimuli. Our results suggest that the presence of early and later inflammatory mediators, such as IL-1β and fibronectin fragments, increases the urokinase system and the matrix metalloproteinases levels. Whereas CRF did not affect this system, VIP counteracts these actions supporting its therapeutic potential for the treatment of osteoarthritis. eng Attribution 4.0 International Urokinase plasminogen activator system in synovial fibroblasts from osteoarthritis patients: modulation by inflammatory mediators and neuropeptides journal article 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URL https://docta.ucm.es/bitstreams/c5e580c9-788d-40ac-9b26-8abb45e28e5f/download File MD5 bf72a867fd10dc0b6ca959e243e42078 405005 application/pdf Urokinase_plasminogen_activator_system.pdf URL https://docta.ucm.es/bitstreams/ccbf0114-dd4a-44cf-a3b8-24880b76a3e6/download File MD5 08c987acca4a840feaf965d55e333119 44843 text/plain Pérez-García et al 2014 J Mol Neurosci uPA.pdf.txt URL https://docta.ucm.es/bitstreams/8b351e1d-78d0-4ba3-aca6-a0bc467402ef/download File MD5 08c987acca4a840feaf965d55e333119 44843 text/plain Urokinase_plasminogen_activator_system.pdf.txt