2026-06-27T10:43:42Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/937362025-03-29T01:12:10Zcom_20.500.14352_14col_20.500.14352_15

Carrión Herrero, Francisco Javier Domínguez Bernal, Gustavo Ramón Martínez Rodrigo, Abel Mas Zubiri, Alicia Blanco Gutiérrez, María Del Mar Orden Gutiérrez, José Antonio Fuente López, Ricardo De La 2024-01-18T07:55:28Z 2024-01-18T07:55:28Z 2017 Domínguez-Bernal, G., Martínez-Rodrigo, A., Mas, A., Blanco, M. M., Orden, J. A., De La Fuente, R., & Carrión, J. (2017). Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells. Comparative immunology, microbiology and infectious diseases, 54, 13–19. https://doi.org/10.1016/j.cimid.2017.07.002 10.1016/j.cimid.2017.07.002 https://hdl.handle.net/20.500.14352/93736 0147-9571 https://doi.org/10.1016/j.cimid.2017.07.002 Here, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection. eng http://creativecommons.org/licenses/by/4.0/ restricted access Attribution 4.0 International Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells journal article