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Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells Carrión Herrero, Francisco Javier Domínguez Bernal, Gustavo Ramón Martínez Rodrigo, Abel Mas Zubiri, Alicia Blanco Gutiérrez, María Del Mar Orden Gutiérrez, José Antonio Fuente López, Ricardo De La 636.09 Dendritic cells HisAK70 Leishmaniosis Salmonella Vaccination Veterinaria 24 Ciencias de la Vida Here, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection. Biodesign Institute Universidad Complutense de Madrid Arizona State University Comunidad de Madrid Ministerio de Economía y Competitividad (España) Depto. de Sanidad Animal Fac. de Veterinaria TRUE pub 2024-01-18T07:55:28Z 2024-01-18T07:55:28Z 2017 journal article VoR https://hdl.handle.net/20.500.14352/93736 XXXX-XXXX 10.1016/j.cimid.2017.07.002 0147-9571 eng Domínguez-Bernal, G., Martínez-Rodrigo, A., Mas, A., Blanco, M. M., Orden, J. A., De La Fuente, R., & Carrión, J. (2017). Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells. Comparative immunology, microbiology and infectious diseases, 54, 13–19. https://doi.org/10.1016/j.cimid.2017.07.002 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ restricted access application/pdf Elsevier