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oai:docta.ucm.es:20.500.14352/937422025-03-29T00:53:34Zcom_20.500.14352_14col_20.500.14352_15

Herrero-Gil, Aldara Carrión Herrero, Francisco Javier Orden Gutiérrez, José Antonio Fuente López, Ricardo De La Domínguez Bernal, Gustavo Ramón 2024-01-18T08:19:09Z 2024-01-18T08:19:09Z 2016 Herrero-Gil, A., Carrión, J., Orden, J. A., de la Fuente, R., & Domínguez-Bernal, G. (2016). Engineering of a live Salmonella enterica serovar Choleraesuis negative-marker strain that allows serological differentiation between immunised and infected animals. Veterinary journal (London, England : 1997), 213, 53–58. https://doi.org/10.1016/j.tvjl.2016.03.016 10.1016/j.tvjl.2016.03.016 https://hdl.handle.net/20.500.14352/93742 1090-0233 https://doi.org/10.1016/j.tvjl.2016.03.016 The usefulness of Salmonella vaccine vehicles is limited by the fact that control programmes relying on Salmonella bacteriology and serology cannot differentiate infected animals from vaccinated ones, an ability referred to as DIVA (differentiating infected from vaccinated animals). As a first step towards Salmonella-based DIVA vaccines, the ompA gene was deleted in live attenuated ΔphoP and ΔrpoS vaccine strains. The ompA gene is present in all Salmonella enterica serovars and it encodes an abundant, highly immunogenic outer membrane protein. The double mutant ΔphoP ΔompA and ΔrpoS ΔompA strains showed similar virulence attenuation, safety and immunogenicity in a mouse model of infection as the parental ΔphoP and ΔrpoS strains. Sera from mice inoculated with the double mutant strains failed to recognise OmpA in Western blots of outer membrane extracts, whereas the protein was recognised by sera from mice inoculated with wild-type Salmonella or a mixture of double mutant and parental strains. These data suggest that OmpA can be a suitable negative marker for DIVA vaccines. eng restricted access Engineering of a live Salmonella enterica serovar Choleraesuis negative-marker strain that allows serological differentiation between immunised and infected animals journal article