2026-06-28T15:18:13Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/940112025-03-29T01:04:47Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Galán-Relaño, Ángela author Gómez-Gascón, Lidia author Casamayor Sanz, Almudena author Luque, Inmaculada author Barrero-Dominguez, Belén author Cardoso-Toset, Fernando author Vela Alonso, Ana Isabel author Fernández-Garayzábal Fernández, José Francisco author Tarradas, Carmen 2024-01-19T10:55:53Z 2024-01-19T10:55:53Z 2019 Galán-Relaño, Ángela, et al. «Antimicrobial Susceptibility and Genetic Characterization of Trueperella Pyogenes Isolates from Pigs Reared under Intensive and Extensive Farming Practices». Veterinary Microbiology, vol. 232, mayo de 2019, pp. 89-95. https://doi.org/10.1016/j.vetmic.2019.04.011. 1873-2542 10.1016/j.vetmic.2019.04.011 https://hdl.handle.net/20.500.14352/94011 1873-2542 https://doi.org/10.1016/j.vetmic.2019.04.011 Trueperella pyogenes is an opportunistic pathogen associated with a variety of diseases and responsible for important economic losses for pig production. Minimal Inhibitory Concentration (MIC) and Pulsed Field Gel Electrophoresis (PFGE) typing analysis were used to determine the MIC distribution and to genetically characterize a total of 180 T. pyogenes isolates obtained from slaughtered pigs reared under intensive (TpIN, n = 89) and extensive (TpEX, n = 91) farming practices. Low MIC90 values for penicillin and amoxicillin (0.008 and 0.06 μg/ml, respectively), ceftiofur, gentamicin and enrofloxacin (1 μg/ml, respectively) were obtained, so they could be of choice for the empiric treatment of T. pyogenes infections. Except for the penicillin, amoxicillin and ceftiofur, a statistically significant difference was observed in the MIC distribution of all antimicrobials analysed between TpIN and TpEX isolates. Also, MIC90 values were higher in TpIN than in TpEX isolates for neomycin and streptomycin (32 μg/ml vs 8 μg/ml), sulfamethoxazole/trimethoprim (30.4/1.6 μg/ml vs 1.90/0.10 μg/ml) and tylosin (≥1024 μg/ml vs 1 μg/ml). A relatively lower genetic diversity was detected in TpIN in comparison with TpEX isolates (GD 0.42 and GD 0.47, respectively). All isolates were distributed in three clusters (A, B, C). TpIN isolates were statistically associated with cluster A (P = 0.0002; OR 3.21; CI95 1.74–5.93), whereas the TpEX were distributed throughout the dendrogram, showing more genetic diversity. These data suggest that the antimicrobial susceptibility and genetic variability of the T. pyogenes isolates could be influenced by the management systems. eng Attribution 4.0 International Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices journal article 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URL https://docta.ucm.es/bitstreams/197f7c1b-5e9f-47a4-a90d-a12fd870c8f2/download File MD5 bce0b6ece2ba82b551465b075bac9912 1079444 application/pdf Antimicrobial_susceptibility.pdf URL https://docta.ucm.es/bitstreams/0d0c7c20-7fda-4e4d-afe0-5dd1163465ab/download File MD5 6d8403cbb58fd1bd9c6143d539d2f536 37767 text/plain Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices.pdf.txt