2026-06-27T23:51:43Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/941032024-09-11T23:47:25Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Rocha Martín, Javier author Martínez-Bernal, Claudio author Pérez-Cobas, Yolanda author Reyes-Sosa, Francisco Manuel author Díez García, Bruno 2024-01-19T14:56:28Z 2024-01-19T14:56:28Z 2017 Rocha-Martín, Javier, et al. «Additives Enhancing Enzymatic Hydrolysis of Lignocellulosic Biomass». Bioresource Technology, vol. 244, noviembre de 2017, pp. 48-56. https://doi.org/10.1016/j.biortech.2017.06.132 0960-8524 10.1016/j.biortech.2017.06.132 https://hdl.handle.net/20.500.14352/94103 https://doi.org/10.1016/j.biortech.2017.06.132 Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila, we studied the effect of different additives on the enzymatic hydrolysis yield. The hydrolysis of pretreated corn stover (PCS), sugar cane straw (PSCS) and microcrystalline cellulose (Avicel) was performed under industrial conditions using high solid loadings, limited mixing, and low enzyme dosages. The addition of polyethylene glycol (PEG4000) allowed to increase the glucose yields by 10%, 7.5%, and 32%, respectively in the three materials. PEG4000 did not have significant effect on the stability of the main individual enzymes but increased beta-glucosidase and endoglucanase activity by 20% and 60% respectively. Moreover, the presence of PEG4000 accelerated cellulase-catalyzed hydrolysis reducing up to 25% the liquefaction time. However, a preliminary economical assessment concludes that even with these improvements, a lower contribution of PEG4000 to the 2G bioethanol production costs would be needed to reach commercial feasibility. eng Additives enhancing enzymatic hydrolysis of lignocellulosic biomass journal article 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URL https://docta.ucm.es/bitstreams/c8733efe-c366-4be6-ba2e-6fa303b7b02c/download File MD5 aa544314255f57d61713f1d5e6166052 1059353 application/pdf Additives_enhancing_enzymatic_hydrolysis.pdf