2026-07-10T21:58:04Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/941122024-09-11T23:52:06Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Trobo-Maseda, Lara
author
Orrego, Alejandro
author
Guisán, José
author
Rocha Martín, Javier
2024-01-19T15:31:47Z
2024-01-19T15:31:47Z
2020
Trobo-Maseda, Lara, et al. «Coimmobilization and Colocalization of a Glycosyltransferase and a Sucrose Synthase Greatly Improves the Recycling of UDP-Glucose: Glycosylation of Resveratrol 3-O-β-D-Glucoside». International Journal of Biological Macromolecules, vol. 157, agosto de 2020, pp. 510-21. https://doi.org/10.1016/j.ijbiomac.2020.04.120.
0141-8130
10.1016/j.ijbiomac.2020.04.120
https://hdl.handle.net/20.500.14352/94112
https://doi.org/10.1016/j.ijbiomac.2020.04.120.
Glycosylation is one of the most efficient biocompatible methodologies to enhance the water solubility of natural products, and therefore their bioavailability. The excellent regio- and stereoselectivity of nucleotide sugar-dependent glycosyltransferases enables single-step glycosylations at specific positions of a broad variety of acceptor molecules without the requirement of protection/deprotection steps. However, the need for stoichiometric quantities of high-cost substrates, UDP-sugars, is a limiting factor for its use at an industrial scale. To overcome this challenge, here we report tailor-made coimmobilization and colocalization procedures to assemble a bi-enzymatic cascade composed of a glycosyltransferase and a sucrose synthase for the regioselective 5-O-β-D-glycosylation of piceid with in situ cofactor regeneration. Coimmobilization and colocalization of enzymes was achieved by performing slow immobilization of both enzymes inside the porous support. The colocalization of both enzymes within the porous structure of a solid support promoted an increase in the overall stability of the bi-enzymatic system and improved 50-fold the efficiency of piceid glycosylation compared with the non-colocalized biocatalyst. Finally, piceid conversion to resveratrol 3,5-diglucoside was over 90% after 6 cycles using the optimal biocatalyst and was reused in up to 10 batch reaction cycles accumulating a TTN of 91.7 for the UDP recycling.
eng
Coimmobilization and colocalization of a glycosyltransferase and a sucrose synthase greatly improves the recycling of UDP-glucose: Glycosylation of resveratrol 3-O-β-D-glucoside
journal article
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https://docta.ucm.es/bitstreams/f79e1630-3b71-4599-a738-65d8d0d5fe90/download
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8e0221e90f0751f9c0906b576ac618fa
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Coimmobilization_and_colocalization.pdf