2026-06-28T10:28:24Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/941312024-04-15T14:36:57Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Sánchez-Jaut, Sandra author Pérez-Benavente, Susana author Abad, Paloma author Méndez Cuadro, Darío author Puyet Catalina, Antonio author Díez Martín, Amalia author Galicia Poblet, Gonzalo author Gómez Domínguez, Elena author Moran Jiménez, María J. author Bautista Santa Cruz, José Manuel author Azcárate, Isabel G. author 2023-02-02 Iron overload caused by hereditary hemochromatosis (HH) increases free reactive oxygen species that, in turn, induce lipid peroxidation. Its 4-hydroxynonenal (HNE) by-product is a wellestablished marker of lipid peroxidation since it reacts with accessible proteins with deleterious consequences. Indeed, elevated levels of HNE are often detected in a wide variety of human diseases related to oxidative stress. Here, we evaluated HNE-modified proteins in the membrane of erythrocytes from HH patients and in organs of Hfe−/− male and female mice, a mouse model of HH. For this purpose, we used one- and two-dimensional gel electrophoresis, immunoblotting and MALDI-TOF/TOF analysis. We identified cytoskeletal membrane proteins and membrane receptors of erythrocytes bound to HNE exclusively in HH patients. Furthermore, kidney and brain of Hfe−/−mice contained more HNE-adducted protein than healthy controls. Our results identified main HNE-modified proteins suggesting that HH favours preferred protein targets for oxidation by HNE. Sánchez-Jaut, S.; Pérez-Benavente, S.; Abad, P.; Méndez-Cuadro, D.; Puyet, A.; Diez, A.; Galicia-Poblet, G.; Gómez-Domínguez, E.; Moran-Jiménez, M.J.; Bautista, J.M.; et al. Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis. Int. J. Mol. Sci. 2023, 24, 2922. https:// doi.org/10.3390/ijms24032922 1422-0067 10.3390/ijms24032922 https://hdl.handle.net/20.500.14352/94131 https://www.mdpi.com/1422-0067/24/3/2922 Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis