2026-06-27T23:05:09Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/942372025-03-18T16:05:32Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Escudero García-Calderón, José Antonio author Rodríguez-Beltrán, Jerónimo author Hernández-Beltrán, J. Carlos R. author De la Fuente, Javier author Fuentes-Hernández, Ayari author MacLean, R. Craig author Peña-Miller, Rafael author San Millan, Álvaro 2024-01-22T08:44:04Z 2024-01-22T08:44:04Z 2018-04-09 2397-334X 10.1038/s41559-018-0529-z https://hdl.handle.net/20.500.14352/94237 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6055991/ Understanding the mechanisms governing innovation is a central element of evolutionary theory. Novel traits usually arise through mutations in existing genes, but trade-offs between new and ancestral protein functions are pervasive and constrain the evolution of innovation. Classical models posit that evolutionary innovation circumvents the constraints imposed by trade-offs through genetic amplifications, which provide functional redundancy. Bacterial multicopy plasmids provide a paradigmatic example of genetic amplification, yet their role in evolutionary innovation remains largely unexplored. Here, we reconstructed the evolution of a new trait encoded in a multicopy plasmid using TEM-1 β-lactamase as a model system. Through a combination of theory and experimentation, we show that multicopy plasmids promote the coexistence of ancestral and novel traits for dozens of generations, allowing bacteria to escape the evolutionary constraints imposed by trade-offs. Our results suggest that multicopy plasmids are excellent platforms for evolutionary innovation, contributing to explain their extreme abundance in bacteria. eng Attribution 4.0 International Multicopy plasmids allow bacteria to escape from fitness trade-offs during evolutionary innovation journal article 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URL https://docta.ucm.es/bitstreams/428b56fb-6eb8-4fd2-94b4-2fbf0da13cb0/download File MD5 4fc64b806c9a385f02da230214b52f1c 2119047 application/pdf emss-76535.pdf URL https://docta.ucm.es/bitstreams/68255c3d-395f-4d58-bed4-6fb0d0d0be92/download File MD5 1b43684650fcc9ba481d52a71112ae83 71146 text/plain emss-76535.pdf.txt