2026-06-27T23:22:39Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/942432024-08-30T08:20:16Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Fuente, Hortensia de la
author
Lamana Domínguez, Amalia
author
Mittelbrunn, María
author
Pérez-Gala, Silvia
author
González, Salvador
author
García-Diez, Amaro
author
Vega, Miguel
author
Sánchez-Madrid, Francisco
editor
Sebastian D. Fugmann
2024-01-22T09:06:34Z
2024-01-22T09:06:34Z
2009
De La Fuente, Hortensia, et al. «Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells». PLoS ONE, editado por Sebastian D. Fugmann, vol. 4, n.o 8, agosto de 2009, p. e6735. https://doi.org/10.1371/journal.pone.0006735.
10.1371/journal.pone.0006735
https://hdl.handle.net/20.500.14352/94243
1932-6203
https://doi.org/10.1371/journal.pone.0006735
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0006735
Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC–mediated immune responses.
eng
Attribution 4.0 International
Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
journal article
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File
MD5
b6adb82889a3b827167bb9b9ea741561
384730
application/pdf
Identification_of_genes.pdf