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oai:docta.ucm.es:20.500.14352/944372025-03-29T00:50:32Zcom_20.500.14352_14col_20.500.14352_15

Pérez Luz, Sara Yañez, María Adela Catalan, Vicente 2024-01-22T15:02:22Z 2024-01-22T15:02:22Z 2004 Pérez-Luz, S., Adela Yáñez, M., & Catalán, V. (2004). Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region. Journal of applied microbiology, 97(1), 191–204. https://doi.org/10.1111/j.1365-2672.2004.02288.x 10.1111/j.1365-2672.2004.02288.x https://hdl.handle.net/20.500.14352/94437 1365-2672 https://doi.org/10.1111/j.1365-2672.2004.02288.x Aim: In this study, we evaluated, the use of universal primers, specific for the 16S-23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water. Methods and results: The analysis of the fragments was carried out using a High Resolution acrylamide/bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro-organisms in samples containing a mixed population. Conclusion: Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates. Significance and impact of the study: A possible new methodology for a rapid identification of pathogens in water. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ restricted access Attribution-NonCommercial-NoDerivatives 4.0 International Identification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region journal article