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00925njm 22002777a 4500 dc Mourino-Alvarez, Laura author Calvo, Enrique author Moreu, José author Padial, Luis author López, Juan author Barderas, María author Gil Dones, Félix author 2013 Background: Circulating endothelial cells (CECs) and endothelial progenitor cells (EPCs) represent two scarce blood populations that are thought to play important roles in tissue vascularization. They have also been proposed as potential markers for more than a dozen pathologies. Moreover, EPCs have arisen as a new therapeutic option for cardiovascular disease. However nowadays there is certain controversy about their roles and a better understanding of EPC biology is required to develop new strategies for forthcoming therapies. Methods: Flow cytometry analysis was performed on freshly isolated mononuclear cells from control subjects and Acute Coronary Syndrome (ACS) patients. EPCs and CECs for both groups were isolated and quantified. Statistical analyses were performed to test the potential biomarker usefulness of both populations in ACS together with the first “in vivo” proteomic characterizations of these populations. Results: Our results do not show statistical differences in the quantification of CECs and EPCs in control subjects and ACS patients. The proteomic characterization allowed us to identify 673 proteins associated to CECs (389 in controls and 462 in ACS patients), and another 502 proteins in EPCs (350 in controls and 274 in ACS patients). Mourino-Alvarez, L., et al. «Proteomic Characterization of EPCs and CECs “in Vivo” from Acute Coronary Syndrome Patients and Control Subjects». Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1830, n.o 4, abril de 2013, pp. 3030-53. https://doi.org/10.1016/j.bbagen.2012.12.014. 0304-4165 10.1016/j.bbagen.2012.12.014 https://hdl.handle.net/20.500.14352/94591 https://doi.org/10.1016/j.bbagen.2012.12.014 Proteomic characterization of EPCs and CECs “in vivo” from acute coronary syndrome patients and control subjects