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oai:docta.ucm.es:20.500.14352/947052024-10-23T15:00:53Zcom_20.500.14352_14col_20.500.14352_15

Detection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular-based diagnostics Castillo, Diana Marcela Sánchez Beltrán, María Del Carmen Castellanos, Jaime Eduardo Sanz Sánchez, Ignacio Mayorga Fayad, Isabel Sanz Alonso, Mariano Lafaurie, Gloria Inés Aim: The aim of this study was to assess the presence of subgingival pathogens in peripheral blood samples from periodontitis patients before and after scaling and root planing (Sc/RP) using nested polymerase chain reaction (nested PCR). Materials and methods: Peripheral blood samples were obtained from 42 patients with severe generalized chronic or aggressive periodontitis. In each patient, four samples of peripheral blood were drawn at different times: immediately before the Sc/RP procedure; immediately after Sc/RP; 15 and 30 min. post-Sc/RP. Blood samples were analysed for bacteraemia with anaerobic culturing and nested PCR, using universal bacterial primers that target the 16S-rRNA gene of most bacteria, subsequently re-amplified with specific primers to Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Eikenella corrodens, Campylobacter rectus and Prevotella intermedia, using a modified phenol-chloroform method for DNA extraction. Results: Presence of specific periodontal pathogens in peripheral blood after treatment was detected in 54.8% of the patients, in 47.6% with anaerobic culturing and in 19% with nested PCR. In 16.6%, the periodontal pathogens were detected before Sc/RP. P. gingivalis and A. actynomicetemcomitans were the pathogens most frequently detected in the bloodstream before and after Sc/RP. Conclusions: Nested PCR demonstrated the presence of DNA from periodontal pathogens in blood samples in severe periodontitis patients before, during and after periodontal therapy. The use of these molecular-based techniques may improve the accuracy from the results obtained by haemoculture. 2024-01-23T11:44:37Z 2024-01-23T11:44:37Z 2024-01-23T11:44:37Z 2011 journal article https://hdl.handle.net/20.500.14352/94705 0303-6979 10.1111/j.1600-051X.2011.01717.x 1600-051X eng Castillo DM, Sánchez-Beltrán MC, Castellanos JE, Sanz I, Mayorga-Fayad I, Sanz M, Lafaurie GI. Detection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular-based diagnostics. J Clin Periodontol. 2011 May;38(5):418-27 http://creativecommons.org/licenses/by-nc/4.0/ restricted access Attribution-NonCommercial 4.0 International Wiley