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A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons Gabilondo, Hugo Losada Pérez, María De La Paloma del Saz, Delia Molina, Isabel León, Yolanda Canal, Inmaculada Torroja, Laura Benito-Sipos, Jonathan 612.82 Neurociencias (Biológicas) 2409.02 Ingeniería Genética This work was supported by a grant from the Spanish Ministerio de Ciencia e Innovación (BFU-2008-04683-C02-02 to L.T.). The central nervous system contains a wide variety of neuronal subclasses generated by neural progenitors. The achievement of a unique neural fate is the consequence of a sequence of early and increasingly restricted regulatory events, which culminates in the expression of a specific genetic combinatorial code that confers individual characteristics to the differentiated cell. How the earlier regulatory events influence post-mitotic cell fate decisions is beginning to be understood in the Drosophila NB 5-6 lineage. However, it remains unknown to what extent these events operate in other lineages. To better understand this issue, we have used a very highly specific marker that identifies a small subset of abdominal cells expressing the Drosophila neuropeptide Capa: the ABCA neurons. Our data support the birth of the ABCA neurons from NB 5-3 in a cas temporal window in the abdominal segments A2–A4. Moreover, we show that the ABCA neuron has an ABCA-sibling cell which dies by apoptosis. Surprisingly, both cells are also generated in the abdominal segments A5–A7, although they undergo apoptosis before expressing Capa. In addition, we have performed a targeted genetic screen to identify players involved in ABCA specification. We have found that the ABCA fate requires zfh2, grain, Grunge and hedgehog genes. Finally, we show that the NB 5-3 generates other subtype of Capa-expressing cells (SECAs) in the third suboesophageal segment, which are born during a pdm/cas temporal window, and have different genetic requirements for their specification. Ministerio de Ciencia e Innovación (España) Depto. de Biología Celular Fac. de Ciencias Biológicas TRUE pub 2024-01-23T14:19:27Z 2024-01-23T14:19:27Z 2011 journal article VoR https://hdl.handle.net/20.500.14352/94807 0925-4773 10.1016/j.mod.2011.01.002 eng BFU-2008-04683-C02-02 Gabilondo, Hugo, et al. «A Targeted Genetic Screen Identifies Crucial Players in the Specification of the Drosophila Abdominal Capaergic Neurons». Mechanisms of Development, vol. 128, n.o 3-4, marzo de 2011, pp. 208-21. https://doi.org/10.1016/j.mod.2011.01.002. open access application/pdf Elsevier